Myogenin Antikörper (C-Term)

Produktnummer ABIN498068

Der Ziege Polyklonal Anti-Myogenin-Antikörper wurde für WB und EIA validiert. Er ist geeignet, Myogenin in Proben von Human, Maus, Ratte, Schwein, Hund und Rind (Kuh) zu detektieren.

Kurzübersicht für Myogenin Antikörper (C-Term) (ABIN498068)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human, Maus, Ratte, Schwein, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Myogenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Myogenin Antikörper

Bindungsspezifität: C-Term

Sequenz: C-TDAHNLHSLT SIVD

Spezifität: Reciognizes Myogenin/MYF4 (C-term).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus (near) of the protein sequence according to NP_002470.2. Genename: MYOG

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/16000. Western blot: 2-4 μg/mL. Detects a band of Approx 38 kDa in Mouse Skeletal Musclelysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: Myogenin

Hintergrund: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4

Molekulargewicht: Human: 25.0kDa (NP_002470.2) Mouse: 25.2kDa (NP_112466.1).

Gen-ID: 4656

NCBI Accession: NP_002470

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development